The lab suggests fixation will lead to the dyes to leak within the cells. I believe the idea is the fact fixation may well destruction cell membranes. I did locate a paper which describes fixation working with methanol or acetic acid, immediately after staining using a DCFDA derivative. DCFDA may be the reagent that stains ROS from the package ab11

The lab says fixation will result in the dyes to leak in the cells. I believe the belief is fixation could damage mobile membranes. I did look for a paper which describes fixation making use of methanol or acetic acid, after staining by using a DCFDA derivative. DCFDA is the reagent that stains ROS in the kit ab113851, and I believe that it's the e